Introduction to Genome assembly
Figure 1
Genome Assembly
Figure 2
Number of genomes in NCBI
Figure 3
Figure 4
Steps Overview
Figure 5
Sequencing Instruments
Figure 6
Challenges in Genome Assembly
Figure 7
Assembly Strategies
Figure 1
Assembly levels
Figure 2
PacBio Assembly
Figure 3
ONT assembly
Figure 4
Hybrid assembly
Figure 5
Figure 6
kmer spectra
Figure 7
Bionano and HiC for assembly improvement
Data Quality Control
Figure 1
Overview of steps
Figure 2
PacBio CCS overview
Figure 3
Overview of ONT read generation
PacBio HiFi Assembly using HiFiasm
Figure 1
HiFiasm assembler
Oxford Nanopore Assembly using Flye
Figure 1
ONT for T2T assemblies
Figure 2
Flye assembly overview
Hybrid Long Read Assembly (optional)
Scaffolding using Optical Genome Mapping
Figure 1
Overview of Bionano OGM
Figure 2
Scaffolding using Bionano
Assembly Assessment
Figure 1
Bandage interface